Effect of MOI on transcriptional profiles of cytokines mRNA after PK-15 cell cultures infected with porcine parvovirus

Abstract:

[Objective] Porcine Kidney-15 cells (PK-15) were infected by Porcine Parvovirus (PPV) to survey the host inflammatory responses, particularly multiplicity of infection (MOI) for the cytokines responses of PPV infection, and the host-PPV interaction. [Methods] Then the viral DNA was measured and analyzed using real-time PCR. The transcript level of cytokines (IFN-β, IRF-3, TNF-α and IL-18) were detected by real-time PCR too. [Results] We found that PPV proliferated rapidly in PK-15 cell 12 hours after infection, and reached peak after 48 hours infection and MOI=1.0; the transcription levels of IFN-β, IRF-3, TNF-α and IL-18 increased obviously, and the transcription level of IRF-3 gene increased to 967 times in 1 hour and MOI=10.0 after infection. [Conclusion] The results revealed that the level of cytokines significantly dependent on the interaction of MOI and infection time.