Identification of degradative bacterium of phenol and its degradation characterization

Abstract:

[Objective] A phenol-degradative strain named T10 which was isolated from the soil sample of a chemical plant was identified, and increased degradative rate of phenol by optimizing the growth conditions. [Methods] The strain was identified by morphological observation, physiological and biochemical properties and 16S rDNA sequence analysis. The phenol-degradative capacity of strain T10 was analyzed when they were cultured in a series of liquid media containing different pH, initial phenol concentration and liquid volume. [Results] The strain T10 belongs to Pseudomonas putida. Glucose and peptone can reduce growth period of strain T10, and the degradation rate of phenol was improved 1.7 times. Under the condition of inoculating concentration 10%, 30 °C and 180 r/min, the phenol removal rate of strain T10 could reach 87.56% when initial phenol concentration, pH and liquid volume were 3?000?mg/L, 7.5, 80 mL/250 mL, respectively. [Conclusion] The experimental data show that strain T10 can tolerate wastewater containing higher concentration phenol, and has stronger degradation ability on phenol. These results would provide reliable basis for further treatment of wastewater containing phenol by the means of biological method.