Isolation, identification and mutation breeding of 3-hydroxypropionic acid high production strain

Abstract:

[Objective] 3-HP is an important bio-based platform compound. We wanted to isolate a strain which can produce 3-HP. [Methods] We isolated samples of soil(17) and feces(10) and did physiological-biochemical identification and 18S rDNA sequence analysis with Y-11 .Then we used UV-NTG-60Coγ to generate mutations. [Results] A yeast Y-11 which can use propionic acid to produce 3-HP was screened by us. It was identified as Candida sp.. We used UV-NTG-60Coγ to generate mutations in Y-11 and got a genetic stability mutant, designated as 5-13B. The mutant 5-13B showed the productivity of 3-hydroxypropionic acid is 11.78 g/L, 2.46 times as that of the primitive strain(Y-11). [Conclusion] The fermentation characters of primitive strain and mutant strain were compared. The results showed that the consumption rate of propionic acid, accumulation of 3-hydroxypropionic acid and tolerance of propionic acid of mutant strain are better than primitive strain.

Key words:Candida sp., 3-Hydroxypropionic acid, Mutagenesis, Fermentation characters