Isolation and identification of pollution endophyte during tissue culture of Dendrocalamus aspera

Abstract:

[Objective] To isolate and identify the endogenous bacteria, which caused tissue culture contamination of Dendrocalamus aspera. [Methods] Using the improved NA culture medium to isolate and identify the strain, then identify the bacterial species by morphology observation, physiological and biochemical tests and the sequence analysis of the 16S rDNA. [Results] The morphological characteristics of the strain SWFU01, together with related physiological and biochemical tests, match the descriptions of Bacillus amyloliquefaciens (Fukumoto) Priest et al.. The sequence analysis of 16SrDNA indicates that this strain has the same embranchment with the Bacillus amyloliquefaciens JS in the Phylogenetic tree, the homology is 99.28%. [Conclusion] By combining the traditional morphology observation and physiological and biochemical tests, the SWFU01 strain is identified as Bacillus amylolique-faciens.