Isolation, identification and bio-detoxification analysis of an aflatoxin B1 degradative strain LAB-10

Abstract:

[Objective] Lactic acid bacterium with aflatoxin B1 detoxification abilities was isolated and evaluated from chicken gut for the present study. [Methods] A special enrichment and isolation technology was designed for aflatoxin B1 detoxified lactic acid bacteria, and the aflatoxin B1 detoxification strain was further determined for its taxonomical position using morphological, physiological and biochemical characteristics combined with phylogenetic analysis. [Results] A lactic acid bacterium strain, which designated LAB-10, displayed aflatoxin B1 detoxification ability was isolated by the present technology. The detoxification analysis results displayed that strain LAB-10 was a aflatoxin B1 degradative bacterium and there was 63.4% of aflatoxin B1 could be decomposed by the strain under the concentration of 14 μg/L, within 48 hours. On the base of taxonomical analysis results, the new isolated strain LAB-10 was identified as Lactobacillus fermentum. [Conclusion] The isolate LAB-10 of the present study was Lactobacillus fermentum and the bio-safety characteristics implied that the degradative stain has potential values for the application of aflatoxins detoxification.