Antifungal susceptibility test of Paecilomyces hepiali by CLSI M38-A2 broth dilution method

Abstract:

[Objective] To test filamentous fungi antifungal susceptibility, this study determined the minimal inhibitory concentration (MIC) values of 6 antifungal agents: Itraconazole (ITC), Ketoconazole (KET), Voriconazole (VRC), Ciclopirox (CIC), 5-Flurocytosine (5FC), Fluconazole (FLU) to the strain Paecilomyces hepiali which usually used in health food industry. [Methods] Method of Clinical Laboratory Standards Institute (CLSI) M38-A2 ＜Reference Method for Broth Dilution Antifungal Susceptibility Testing of Filamentous Fungi; Approved Standard—Second Edition＞ was referred. [Results] The results showed that the quality control strain Candida parapsilosis ATCC 22019T MIC values were within the reference range, the strain P. hepiali MIC values for the 6 antifungal agents were 1 mg/L, 0.5 mg/L, 0.25 mg/L, ≥128 mg/L, 64 mg/L, 2 mg/L respectively. [Conclusion] M38-A2 method could be applied to determine the susceptibility of P. hepiali to antifungal agents. But, more tests needed to acquire the standard interpretation of the strains susceptibility.

Key words:Paecilomyces hepiali, Susceptibility test, CLSI M38-A2