Determination of Coxiella burnetii in egg yolk by PCR method

Abstract:

[Objective] Improve the detection rate of Coxiella burnetii (C. b) Com1 gene by quantitative PCR and nested PCR; determine the food safety of egg yolk by Coxiella burnetii Com1 gene detection, which has great magnificence for Coxiella burnetii epidemiology. [Methods] Extract DNA in chicken eggs, determine the Com1 gene by quantitative PCR and nested PCR, and sequence the PCR products. Observe the microorganism in chicken blood leukocytes by indirect immunofluorescence. [Results] Results showed that over 4 Com1 genes were detected by quantitative PCR and nested PCR, and the amount of Com1 genes in chicken eggs reached 104?106 with the positive rate of 5%?22%. The sequencing results of PCR products showed that there were variant strains in positive chicken eggs. Immunofluorescence observed the microorganism Coxiella burnetii in chicken eggs. [Conclusion] Therefore we draw the conclusion that the microorganism Coxiella burnetii exists in chicken eggs and it may be the source of infection of Q fever.

Key words:Coxiella burnetii, Quantitative PCR, Nested PCR, Chicken egg