Influence of low pH on the adhesion ability of Bifidobacterium bifidum KLDS2.0603

Abstract:

[Objective] The influence of low pH on the adhesion ability and the surface properties of Bifidobacterium bifidum KLDS2.0603 were determined in this paper. [Methods] After Bifidobacterium bifidum KLDS2.0603 was treated by PBS of different low pH, the adhesion ability was assayed by gram staining microscopic examination and plate colony counting method. The autoaggregation ability and surface hydrophobicity of Bifidobacterium bifidum KLDS2.0603 were also assayed. [Results] The adhesion abilities of Bifidobacterium bifidum KLDS2.0603 treated by PBS with different low pH were decreased. The adhesion abilities of treatment groups were significantly lower than control group except the group of pH 5.0. The surface hydrophobicity of Bifidobacterium bifidum KLDS2.0603 treated by PBS of pH 3.0 and 3.5 was significantly increased. Except for experimental groups of pH 1.0, 1.5 and 5.0, the autoaggregation ability of Bifidobacterium bifidum KLDS2.0603 was significantly reduced. [Conclusion] The adhesion ability of Bifidobacterium bifidum KLDS2.0603 was reduced in the low pH environment. The autoaggregation ability and surface hydrophobicity of Bifidobacterium bifidum KLDS2.0603 were also affected. The adhesion ability was positive correlation with the autoaggregation ability and surface hydrophobicity excluding the groups of the pH 3.0 and 3.5 for Bifidobacterium bifidum KLDS2.0603.