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    Abstract:

    [Objective] To identify the black yeast-like fungus isolated from the radiation-polluted soil and investigate the resistance. [Methods] the colony morphology and mycelium of isolates was observed, and phylogenetic tree was constructed based on LSU rDNA D1/D2 domain sequences. And then, the isolates resistance to 60Co gamma rays and ultraviolet rays and stress resistance to heavy metals and salt were analyzed. [Results] The results showed that isolates were varieties of Aureobasidium pullulans. The isolates F99 and F134 had a high similarity (100%) with A. pullulans var. subglaciale CBS 123388T as the closest relative, and the isolate F19 had a high similarity (100%) with A. pullulans var. melaogenum CBS 105.22T as the closest relative. Furthermore, isolates had higher radiation resistance and stress resistance. [Conclusion] This paper provides important materials to investigate the mechanism of radiation-resistant fungus.

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ZHANG Zhi-Dong, XIE Yu-Qing, WANG Wei, GU Mei-Ying, ZHU Jing, TANG Qi-Yong, SONG Su-Qin, SHI Yu-Hu. Isolation and character of radio-resistant black yeast-like fungus[J]. Microbiology China, 2012, 39(5): 0724-0731

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  • Online: June 07,2012
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