Proteomic analysis of effects of iron depletion on Streptococcus pyogenes MGAS5005

Abstract:

[Objective] Analyze the effects of iron deficiency on Streptococcus pyogenes, and find the key proteins in the iron acquisition systems. [Methods] Streptococcus pyogenes was cultured in THY medium with or without iron. Total proteins were extracted for the 2DE-gel analysis. The altered proteins upon iron depletion were identified by mass spectrometry. Further bioinformatics analyzed the relationship between key differentially expressed proteins. [Results] The 20 alterated protein were identified. With Cytoscape analysis of the betweenness of these proteins, 5 bottleneck proteins were detected. [Conclusion] The results showed that iron depletion affect the cellular biosynthetic processes, the metabolic process of compound containing nitrogen and the macromolecular metabolic process etc. This study laid a foundation for further studying the mechanisms of the iron metabolism in bacteria.