Chlorella high density training and lipid extraction condition optimizing

Abstract:

[Objective] Chlorella high density training and lipid extraction condition optimizing. [Methods] Single factor experiments were used to research different mediums and environmental factors on Chlorella cell growth effects, and the ultrasonic extraction method was employed by orthogonal experiment for algae powder oil extraction conditions. [Results] The optimal condition of Chlorella ellipsoidea Y4 under heterotrophic culture to get high biomass were: BG11 medium with 50 g/L of glucose as the carbon source and 2 g/L KNO3 as the nitrogen source. The optimum culture temperature, shaking rate and the inoculums size were 29 °C, 180 r/min and 20%. By inoculating the preculture to a fermentation tank of 1 L capacity, we got dry cell weight 18.25 g/L. Based on the oil extraction condition optimization, Y4 lipid extraction yield increased from 25% to 60.2%, with lipid extraction yield raised by 35.2%. [Conclusion] Optimization of chlorella culture conditions and lipid extraction conditions were studied, and the result promoted the exploitation and utilization of chlorella resources.