Isolation and identification of the causative agent and histopathology observation of white-spots disease in internal organs of Larimichthys crocea

Abstract:

[Objective] In order to find the pathogenic bacteria of white-spots disease in internal organs of Larimichthys crocea. [Methods] Using the method of isolation and identification of the causative agent in combination with 16S rRNA sequencing to find the pathogenic bacteria.Besides it was determined by artificial infection. The tissue of diceased fish were pathologically analyzed by paraffin histological section techniques. [Results] The bacterium Pseudomonas putida was responsible for the disease. Affected fish showed obvious inflammation and cell deformation or disintegration of the liver, kidney and spleen. [Conclusion] The conducted study was necessary in order to reveal the reason for the high mortality of cultured yellow croaker in the Xiangshan bay. Furthermore, the results of this study can help to early recognize such disease outbreaks, hence preventing drastic economic losses in the future.