Molecular cloning and immunological characterization of SSU2100 gene from srtBCD pilus island of Streptococcus suis 2

Abstract:

[Objective] To investigate the immunoprotection of pilus subunit SSU2100 which was encoded by srtBCD pilus island presented in the Chinese strain 05ZYH33 of S. suis 2. [Methods] The SSU2100 gene was amplified by PCR, then cloned into prokaryotic expression plasmid pET28a, and expressed the recombinant SSU2100 in E. coli BL21. The SSU2100 protein was purified by affinity chromatography and analyzed by Western blotting. The mice anti-SSU2100 serum was prepared by immunizing mice with recombinant SSU2100 protein and analyzed by ELISA. [Results] The high titer of the serum were obtained, Western blot result indicated that the recombinant protein can react with mice anti-SSU2100 serum. Animal test showed that vaccinating mice with recombinant SSU2100 protein conferred a significant protection. [Conclusion] SSU2100 could serve as a vaccine candidate of S. suis 2, it provides the foundation for system-atically illustrating the role that srtBCD pilus island plays in pathogenicity of S. suis 2.