Screening and identification of cold-active amylase strain C2 and its amylase characterization

Abstract:

Strain producing cold-active amylase want to be isolated. Enzymatic properties of the strain will be determined. Screening strains from The Black mountant. Determine its species by its morphological, the physiological and biochemical properties and 16S rDNA sequence analysis, to study its enzymatic properties. A strain C2 producing cold-active amylase was identified as Exiguobacterium sp.. Enzymatic properties showed that the optimum pH value is 7.5 and the optimum reaction temperature is 25 °C, but the thermal stability of the enzyme is relatively poor. The amylase activity was activated by Ca2+ and Fe2+, and was inhibited by Cu2+, Ni2+ and Go2+. By the identification of TLC, the product of enzymolysis is glucose. It is indicates that the strain can product cold-active starch glucoamylase. Amylase produced by strain C2 in line with the nature of cold-active amylase, it deserves to be further researched.