The structure and performance testing of a new kind of mucopolysaccharide

Abstract:

Mucopolysaccharide was made of uronic acid and hexosamine. Due to the unique physical and chemical properties, mucopolysaccharides have been used in various fields. After collecting and enrichment culture of active nasal mucosa scraped from newly slaughtered cattle, we got a kind of streptococcus zooepidemicus which was named BU100. With its culture it can secrete a high yield new Mucopolysaccharide A. As there were uronic acid and hexosamine in mucopolysaccharide, the methods of carbazole and Elson-Morgan were used to detect mucopolysaccharide A. In addition, the major impurity (protein) in mucopolysaccharide was detected by the method of Coomassie brilliant blue. According to these methods, it was found that the ratio of uronic acid and hexosamine in mucopolysaccharide A was nearly 1:1 which was the same as that in Hyaluronic Acid (HA), and the quantity of protein meets the standards (<0.1%) requirements. Furthermore with the IR, NMR, the functional group of mucopolysaccharide A was determined and compared to HA. It therefore indicates that most structure and functional group in mucopolysaccharide A were nearly as the same as that in HA. As there were uniqe properties in mucopolysaccharide A, a lot of properties were detected, such as moisture absorbtion, moisture retention, reducing power, clearance of hydroxyl radical, resistance of hyaluronidase. These properties of mucopolysaccharide A were also compared with that of HA. The results showed that the clearance of hydroxyl radica, moisture retention, and reducing power of mucopolysaccharide A was better than HA, but the absorbtion rate of mucopolysaccharide A was not as well as that of HA, and mucopolysaccharide A could resist to Hyaluronidase. Because the structure and functional group of mucopolysaccharide A was highly similar to HA, meanwhile some characters of mucopolysaccharide A were superior to HA, the conclusions was that mucopolysaccharide A can be more effectively applied in medical, cosmetics, etc.