Optimization extraction methods of polysaccharide of chlamydospores wall in Ustiloginoidea virens

Abstract:

To reveal the content and composition of polysaccharide in chlamydospore wall of Ustiloginoidea. virens, a study of the optimum method to extract polysaccharide of the chlamydospore wall was conducted. Five different methods were adopted to extract polysaccharide of its black chlamydospore wall, and the contents of polysaccharide were determined by phenol-sulfuric acid method. The results showed that the optimum extracting method was the complex enzyme-hot water extraction-sevag and the most appropriately extracting conditions were that the complex enzyme, pH 4 extracting tempera-ture extracting time and the material ratio were 4%, 70 °C for 120 min, 1:75 (V/V), respectively. Based on above the optimum method, the relatively crude polysaccharide and polysaccharides rates in the black and yellow chlamydospore wall were separately 21.2% and 72.3%, and 17.5% and 66.7%. Con-sequently, the complex enzyme-hot water extraction-sevag and its optimizing conditions among the five different methods were simple, efficient and particularly suitable to the determination polysaccharide in chlamydospores wall of U. virens.

Key words:Rice, Ustilaginoidea virens, Chlamydospores, Polysaccharide