The establishment of LAMP rapid detection method for Clostridium perfringens in food

Abstract:

LAMP (Loop-mediated isothermal amplification, LAMP) rapid detection method for Clostridium perfringens in food was established, with the specific primers designed on the CPa gene. The results of the study indicated that the method had perfect specificity, as 5 strains of Clostridium perfringens were able to amplify specific fragments, while 13 strains of non-Clostridium perfringens were not, and no false positive or false negative results occurred. The test could be done within 1 hour with the sensitivity low to 10 fg/μL. The established method provides a better choice for rapid detection of Clostridium perfringens.