Isolation and identification of a parathion-methyl degrading fungus-Aspergillus oryzae JMUPMD-2

Abstract:

A newly isolated fungus JMUPMD-2, capable of growing with parathion-methyl as the sole phosphorus source, was identified based on morphological charaterisitics and rDNA ITS sequences analysis, and its capability of degrading parathion-methyl was investigated by determining the changes of parathion-methyl concentration in the broth of the isolate. JMUPMD-2’s colonic and microscopic morphologies were identical with and its sequences of rDNA ITS was 99% similar to Apspergillus oryzae. Consequently, it was identified as Aspergillus oryzae. When cultured with an artificial medium containing parathion-methyl as the sole phosphorus or parathion-methyl-K2HPO4 mixed phosphorus, concentration of parathion-methyl respectively reduced 189 μg/L and 28 μg/L after inoculating for 200 hours. JMUPMD-2’s intracellular extract had obvious parathion-methyl degrading enzyme activity.