Influence of Raphanus sativus L. on the mycelium growth of Cenococcum geophilum Fr.

Abstract:

The influence of radish (Raphanus sativus L.) seeds, seedlings, root exudates, and seedling extracts on the mycelium growth of Cenococcum geophilum Fr. (Cg) was investigated in vitro. The mycelium growth of Cg strains (CgSO1, CgSB2, CgO5, SPOP2 and Cg5#) was promoted, when Cg strains were co-cultured with radish seedlings, or cultured with root exudates, and water extract of radish seedlings. However, Cg mycelium growth was significantly improved by the autoclaved (treated under high temperature) root exudates or autoclaved water extract of radish seedlings, while the autoclaved seedlings segments did not. Results showed that the maximum mycelium dry biomass of the 5 strains of Cg was given in the medium added the autoclaved water extract of the seedlings. The single colony dry maximum biomass of each Cg strain (CgSO1, CgSB2, CgO5, SPOP2 and Cg5#) was reached to 54.8, 45.8, 63.9, 41.2 and 50.5 mg respectively.