Characterization of fungal morphology based on the combination of macroscopic and microscopic view in steroid microbial hydroxylation

Abstract:

To research the effects of growth regulator on the status of mycelia growth during steroid biotransformation, morphology of filamentous microorganisms was characterized in combination with both sedimentary density method in macroscopic view, and using Scanning Electron Microscope (SEM) method in microscopic view during the whole strain growth phases. The results showed that in comparison with strain growing without adding growth regulator, the strains growing, which was added growth regulator, showed relatively lower sedimentation densities during all different stages than the one not added. Through SEM, it can be clearly seen that the bacteria with the regulator presents better conditions such as smooth surfaces, rounded plump as well as excellent shapes. Both methods prove adding regulator are able to promote the exuberant growth of the bacteria.