Detection of autoinducer in Vibrio qinghaiensis sp. -Q67 and its effect on luminescence

Abstract:

Vibrio qinghaiensis sp. -Q67 was a newly identified luminescent bacterium in fresh water, its character of luminous intensity changed with toxic pollutant concentrations made it as an strain for water quality monitoring. Through bioassay strain JZA1 test, C18 reverse phase thin-layer chromatography and LacZ activity test, it was identified that Q67 had a LuxI-LuxR type Quorum-Sensing and produced N-acyl-homoserine lactone (AHL) as autoinducer. The other test results indicated that the content and activity of the signal molecular in Q67 changed with its growth phases. The crude extract from Q67 affected not only its luminescence but also its growth and reproduction.