Screening and identification of a Bacillus cereus strain producing fibrinolytic enzyme from earthworm

Abstract:

One hundred and twenty-two bacteria strains were isolated from the intestinal tracts and body surface of five earthworms collected from four different provinces. Twelve fibrinolytic enzyme producing strains were screened based on the size of halo zone by using powdered skim milk and fibrous protein as the first and second round screening substrates, respectively. SC-3-W-3 strain with high fibrinolytic enzyme activity was identified as Bacillus cereus based on physiological and biochemical characteristics, as well as 16S rDNA. The fibrinolytic enzyme activity of SC-3-W-3 strain was up to 538.64 U/mL.