Screening of new ε-polylysine producing strain and structure identification of its product

Abstract:

A novel ε-polylysine (ε-PL) producer with 0.846 g/L yield, designated strain Str-8, was screened from the soil collected from Guangdong, China, by using Nishikawa’s method with some improvement. Taxonomic identification of the strain, including morphology, culture characteristics, physiological and biochemical characters were performed and phylogenetic tree was constructed based on the 16S rDNA sequences, which indicated that it belongs to Streptomyces ahygroscopic. The purified fermentation product of this strain was identified as ε-PL by characteristic analysis, hydrolysate analysis, mass spectrum and UV spectrum.