Plasmid elimination method and conditions of Burkholderia cepacia T1828

Abstract:

To explore the suitable for Burkholderia cepacia plasmid elimination method and study the optimal conditions for plasmid elimination, we eliminated plasmid of Burkholderia cepacia T1828 by using sodium dodecyl sulfate and improving the growth temperature combination method, UV-coated plate method, freeze-thaw method, acridine orange method and baicalin extraction methods such as elimination methods. The results showed that sodium dodecyl sulfate and improving the growth temperature combination method is best suited to Burkholderia cepacia T1828 plasmid elimination, while the best conditions for the elimination are T1828 culture after 16 h by adding SDS to 0.1% of the final solubility dealt with 18 h at 36 °C, the elimination rate reach 49%. The plasmid-free mutant strains can be used for further study.