Isolation and catalytic characteristics of a glycolonitrile-hydrolyzing strain

Abstract:

A strain CCZU-12 with high glycolonitrile-hydrolyzing activity has been isolated using glycolonitrile as sole nitrogen source. It was identified as Pseudomonas sp. by its morphological, physiological properties and 16S rDNA sequence analysis. The preferred carbon/nitrogen sources and metal ions were sodium acetate (10 g/L), yeast extract (5 g/L), and Mg2+ (1.0 mmol/L), respectively. The optimum culture conditions were the filling volume 50 mL in 250 mL shaking flask, inoculum 4%, temperature 30 °C, and initial pH 7.0. After hydrolysis of glycolonitrile for 120 h, glycolic acid was obtained in a yield of 98.9% at the optimum reaction temperature 35 °C and pH 7.0.