Isolation, identification and fermentation characterization of a 1,3-propanediol product bacterial

Abstract:

A bacterial strain named as 2-1 was isolated from activated sludge, which can product 1,3-propanediol (1,3-PD) from glycerol. Morphologic properties, physiological and biochemical characteristics and 16S rRNA gene sequence analyses were used to identify the strain. The phylogenetic analysis of the 16S rRNA sequence indicated that the strain 2-1 closely with Klebsiella pneumoniae (CP001891), whereas, the homologous rate was 95.4%. The strain 2-1 might represent a new species. In the fed-batch fermentation at 5 L fermentor: the maximum concentration of 1,3-PD was 63.5 g/L; productivity was 2.19 g/(L·h); substrate conversion rate was 0.64 mol/mol.