The reduction and detoxification to selenite by Rhodopseudomonas palustris

Abstract:

The reduction and detoxification mechanism of sodium selenite by Rhodopseudomonas palustris were studied in this paper. Effect of factors on the reduction and detoxification of sodium selenite based on a single-factor test and orthogonal experiment were investigated. Results showed that selenite removal rate by the reduction and detoxification of Rhodopseudomonas palustris were achieved at 98.2% under optimal conditions with concentration of sodium selenite at 25 mg/L, 15% of inoculums amount (W/W) and cultivation for 5 days. The researches indicated that selenite reductase with the molecular weight of 182 kD was composed of four subunits polymers and distributed in cytoplasm. Reducing selenite into selenium particles with diameter in 5 nm?200 nm during reduction and detoxification of Rhodopseudomonas palustris were also primarily identified by transmission electron micrograph.

Key words:Rhodopseudomonas palustris, Selenite, Reduction and detoxification, Orthogonal experiment, Reductase