Efficient degradation of polyporus fungus Trichaptum abietinum 1302BG on the synthetic dyes congo red and fuchsin acid under non-sterile condition

Abstract:

Soil samples collected from bamboo agroforestry fields in Hangzhou, China, were studied for screening and isolation of fungal strains capable of degradation of structurally different dyes. Among all isolates, an isolate (1302BG) could decolorize all of the 9 tested dyes (Aniline blue, Congo red, Orange G, Methyl red, Methyl orange, Crystal violet, Fuchsin acid, Safranine T, Fuchsin basic, and Methyl violet) on the solid agar plates. The isolate was identified as Trichaptum abietinum by morphological and molecular methods. Different parameters such as pH, temperature, carbon source, nitrogen source, co-effect of carbon and nitrogen source, and carbon/nitrogen regimes were used to find an inexpensive medium for Trichaptum abietinum 1302BG to decolorize. The results showed that the strain was very efficient at decolorizing the two tested dyes (Congo red and Fuchsin acid) in both acid and basic conditions. The inexpensive and environment-friendly medium contained 0.5 g/L starch and 0.05 g/L ammonium sulfate and could decolorize the two dyes over 90% after 24 hours incubation under both sterile and non-sterile (nature) conditions. The UV-vis absorption spectrum indicated that the decolorization by this fungus should be attributed to biodegradation. A great decrease in toxicity after the decolorization of Congo red and Fuchsin acid was observed by micronucleus tests. The results suggest that Trichaptum abietinum 1302BG is a highly promising fungus for the treatment of industrial dye effluents.