Isolation, identification and enzymatic characteristics of cellulose-producing strains with high cellulase activity

Abstract:

Two cellulose producing strains were isolated from decayed leaf and soil around it. According to morphology, biochemical and physiological characterization, and 16S rRNA sequence analysis, CT1 was identified as Bacillus licheniformis and CM2 was identified as Bacillus subtilis. Through deteminations of CMC enzyme activity and filter paper enzyme activity using liquid fermentation. The result of the CMCasae activity of CT1 is 163.3 U/mL and CM2 is 167.17 U/mL after 4 d, the FPA activity of CT1 is 211.17 U/mL after 2 d and CM2 is 207.83 U/mL after 3 d. The effect of carbon sources on the optimum cellulase-producing conditions of these strains were studied. By silver staining after SDS-polyacrylamide gel electrophoresis preliminary separation cellulose enzyme special bands. The result showed that the utilization capabilities to different source cellulose were different.