Domestication for imidacloprid-resistant strain of rice brown planthopper, <em>Nilaparvata lugens</em> St?l, symbiote, <em>Candida lipolytica</em>

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Domestication for imidacloprid-resistant strain of rice brown planthopper, Nilaparvata lugens St?l, symbiote, Candida lipolytica
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                        LI NaLI Na
State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang 310021, China; College of Life and Environment Sciences, Ha
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CHEN Jian-MingCHEN Jian-Ming
State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang 310021, China
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ZHANG Jue-FengZHANG Jue-Feng
State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang 310021, China
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HE Yue-PingHE Yue-Ping
State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang 310021, China
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CHEN Lie-ZhongCHEN Lie-Zhong
State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang 310021, China
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Abstract:

To further study physiological-biochemical mechanism of symbiote in imidacloprid-resistant brown planthopper (Nilaparvata lugens), imidacloprid-resistant strain of symbiote, Candida lipolytica, was domesticated with different concentrations of imidacloprid based on effect of insecticides on growth and development of C. lipolytica. The results indicated that after in vitro C. lipolytica, on solid culture medium containing 2 000, 1 000 and 500 mg/L imidacloprid were subcultured for continuous 20 generations, the colony numbers of C. lipolytica on solid culture medium containing 2 000 mg/L imidacloprid were no significant difference with those on control medium containing non-imidacloprid. When numbers of colony kept stable for continuous three generations, the domesticated strain was called 2 000 mg/L imidacloprid- resistant one. Morphological changes of imidacloprid -resistant and -susceptible strains pseudohyphae were further observed using light microscope, it was found that pseudohyphae of imidacloprid- resistant strain occurred malformation and become shorter, some appeared enlargement.

Key words:Nilaparvata lugens, Candida lipolytica, Imidacloprid, In vitro culture, Domestication of imidacloprid- resistant strain

Reference

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LI Na, CHEN Jian-Ming, ZHANG Jue-Feng, HE Yue-Ping, CHEN Lie-Zhong. Domestication for imidacloprid-resistant strain of rice brown planthopper, Nilaparvata lugens St?l, symbiote, Candida lipolytica[J]. Microbiology China, 2011, 38(2): 237-241

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Received:September 11,2010
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