A bacterial endophyte from banana: its isolation, identification, activity to Fusarium Wilt and PGPR effect to banana seedlings

Abstract:

Strain DB09208, an endophytic bacterium exhibited strong inhibitory activity in vitro against Fusarium oxysporum f. sp. cubense FS-0704 (race 4: FOC4) was isolated from Musa AAA, cv. Baxi grown in Hainan. Based on the morphological, physiological and biochemical properties, and the 16S rDNA sequence analysis, this strain was identified as Paenibacillus sp.. The strain DB09208 obviously inhibited the mycelial growth of FS-0704, with an inhibitory rate of 74.09%, pot experiments indicated the strain suppressed Fusarium wilt of bananas, the control efficiency reached 65.2%. It could significantly promoted the generation of roots with the effect was similar to that of IAA. These demonstrated that strain DB09208 should be useful in biological control bananas Fusarium wilt.

Key words:Fusarium wilt, Endophytic bacteria, 16S rDNA, Isolation and identification