PCR-SSCP analysis bacterial community of Mud Volcano in Xinjiang

Abstract:

A technology sigle-strand conformation polymorphism (SSCP) and a target 16S rDNA gene on V3 region were used to analyze mud volcano microbial community and structure. After extracting DNA samples from different mud deepth and month, get a 236 bp fragment by PCR. The fragment was used to analyze the microorganisms seasonal diversity of mud volcano by SSCP, and some target fragments were analyzed clone. Resluts showed: the bacteria polymorphism in Xinjiang mud volcano soil is significant, which is vulnerable to ecological and climatics factors. Pseudomonas is a dominant microorganism in Xinjiang mud volcano, it distributes widely in mud volcano and was affected little by ecological and climatics.

Key words:Mud volcano, PCR-SSCP, 16S rDNA