Screening and identification of a cold-active endoglucanase-producing strain and its endoglucanase characterization

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Screening and identification of a cold-active endoglucanase-producing strain and its endoglucanase characterization
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                        DONG ShuoDONG Shuo
Bioengineer College, Dalian University, Liaoning Technology of Marine Microbiological Engineering Research Center, Dalian, Liaoning 116622, China
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CHI Nai-YuCHI Nai-Yu
Bioengineer College, Dalian University, Liaoning Technology of Marine Microbiological Engineering Research Center, Dalian, Liaoning 116622, China
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ZHANG Qing-FangZHANG Qing-Fang
Bioengineer College, Dalian University, Liaoning Technology of Marine Microbiological Engineering Research Center, Dalian, Liaoning 116622, China
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A strain SWD-28 producing cold-active endoglucanase was isolated from samples of Changhai county in Yellow Sea. It was identified as Penicillium cordubense based on the morphological and ITS sequence. An endoglucanase was isolated and purified from it. The specific activity of endoglucanase was 26.4 U/mg increased 20.6 fold and coefficient of recovery was 13.1%, the molecular mass was 33.1 kD. Analysis by circular dichroism (CD) spectrum of purified endoglucanase protein revealed that it displayed typical α-helix structure and α-helix 49.9%, β-sheet 0.0%, turn 24.3% and random coil 25.8%. Enzymatic properties showed that the optimum pH value is 5.0 and the optimum reaction temperature is 35 °C, at 5 °C the relative enzyme activity still reach to 60%.

Key words:Cold-active endoglucanase, Penicillium cordubense, Purification, Enzymatic properties

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DONG Shuo, CHI Nai-Yu, ZHANG Qing-Fang. Screening and identification of a cold-active endoglucanase-producing strain and its endoglucanase characterization[J]. Microbiology China, 2011, 38(2): 169-175

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Received:September 25,2010
Revised:November 24,2010
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