Preliminary study of effects of matrine on Staphylococcus epidermidis biofilms

Abstract:

A new therapeutic approach for Staphylococcus epidermidis biofilm-associated infections was provided by the study of active ingredient of Chinese medicine matrine on S. epidermidis biofilm inhibition. XTT reduction assay was used to evaluate matrine on the initial adhesion of S. epidermidis and biofilm metabolism, and microscope was applied to observe biofilm morphology. The results showed that SMIC₅₀ and SMIC₈₀ of matrine against S. epidermidis biofilms were 62.5 mg/L and 500 mg/L respectively, 1 000 mg/L of matrine inhibited the early adherence of S. epidermidis, and 250 mg/L of matrine had a significantly inhibitory effect on S. epidermidis biofilm morphology. The study demonstrated that matrine could inhibit S. epidermidis biofilm formation and adhesion.

Key words:Biofilm;Staphylococcus epidermidis;Matrine