Isolation and identification of endophytic fungi with antimicrobial activity from Fagopyrum dibotrys and Fagopyrum tataricum

Abstract:

A total of 62 endophytic fungi were isolated from the plant tissues (root, stem, leaf and flower) of medicinal plants Fagopyrum dibotrys and Fagopyrum tataricum. The antimicrobial activity from isolated endophytic fungi were detected using agar diffusion method with Staphylococcus aureus, Es-cherichia coli, Bacillus subtilis [CMCC (B) 63501], Fusarium graminearum, Fusarium oxysporum f. sp. cucumerinum and Pythium aphanidermatum as indicator microorganisms. The results showed that the ethanol extracts of three isolated entophytic fungi strain KQH-01, KQH-02 and JQY-1 had relatively strong antibiotic activity to some indicator microorganisms. By using morphological and molecular identification, strain KQH-01was determined to belong to the genus Xylaria sp., and strains KQH-02 and JQY-1 were identified as Chaetomium globosum and Botryosphaeria dothidea respectively.

Key words:Fagopyrum dibotrys;Fagopyrum tataricum;Endophytic fungi;Antimicrobial activity;Molecular identifi-cation