A Experiment Module of Isolation, Cultivation and Breeding of Protease-producing Bacteria for Microbiological Experiment Teaching

Abstract:

We integrated a group of related experiments focusing on isolation, cultivation and mutation breeding of protease-producing bacteria into an experiment module and applied it in microbiological experiment teaching. The experiment module included preparation and sterilization of media, isolation of protease-producing bacteria, optimization of cultivation conditions, characterization of the strain, mutation breeding of the strain by UV exposure.