Mutation and Selection of Transglutaminase Producing Strain by Atmospheric Pressure Glow Discharge Plasma

Abstract:

A novel mutation approach, namely, the atmospheric pressure glow discharge (APGD) plasma jet driven by a radio frequency (RF) power, was used to treat the spores of Streptoverticillium mobaraense 03-10 for the selection of transglutaminase (TGase) producer. The mutant with high TGase production was quickly screened according to the formation of color on the double-layered plate and the different appearances of colonies. The total mutation rate was over 42.8% and the positive mutant rate was 20.6%. The obtained mutant G2-1 has good genetic and morphological stability and TGase activity reached 2.73 U/mL, which was higher 82% than that of original strain.