- LI Xiao-Jiao
LI Xiao-Jiao
1. Key Laboratory of Genetic Function and Regulation, College of Bioengineering, Chongqing University, Chongqing 400030, China
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Search for this author on this site - WANG Zhong-Kang
WANG Zhong-Kang
1. Key Laboratory of Genetic Function and Regulation, College of Bioengineering, Chongqing University, Chongqing 400030, China
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Search for this author on this site - ZHANG Gui-Ming
ZHANG Gui-Ming
2. Technical center of APIQ, Shenzhen Entr-Exit Inspection and Quarantine Bureau, Shenzhen 518045, China; 3. Shenzhen Key Laboratory for Research & Development on Detection Technology of Exotic Pests, Shenzhen 518045, China
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Search for this author on this site - CHEN Zhi-Nan
CHEN Zhi-Nan
2. Technical center of APIQ, Shenzhen Entr-Exit Inspection and Quarantine Bureau, Shenzhen 518045, China; 3. Shenzhen Key Laboratory for Research & Development on Detection Technology of Exotic Pests, Shenzhen 518045, China
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Search for this author on this site - YIN You-Ping
YIN You-Ping
1. Key Laboratory of Genetic Function and Regulation, College of Bioengineering, Chongqing University, Chongqing 400030, China
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Search for this author on this site - CHENG Ying-Hui
CHENG Ying-Hui
2. Technical center of APIQ, Shenzhen Entr-Exit Inspection and Quarantine Bureau, Shenzhen 518045, China; 3. Shenzhen Key Laboratory for Research & Development on Detection Technology of Exotic Pests, Shenzhen 518045, China
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Search for this author on this site - WANG Ying
WANG Ying
2. Technical center of APIQ, Shenzhen Entr-Exit Inspection and Quarantine Bureau, Shenzhen 518045, China; 3. Shenzhen Key Laboratory for Research & Development on Detection Technology of Exotic Pests, Shenzhen 518045, China
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Search for this author on this site - XIANG Cai-Yu
XIANG Cai-Yu
2. Technical center of APIQ, Shenzhen Entr-Exit Inspection and Quarantine Bureau, Shenzhen 518045, China; 3. Shenzhen Key Laboratory for Research & Development on Detection Technology of Exotic Pests, Shenzhen 518045, China
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Search for this author on this site - MIU Jian-Kun
MIU Jian-Kun
4. China Agricultural University, Beijing 100094, China
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Two standard molecules were constructed by molecular clone technology of Tilletia indica Mitra and Tilletia walkeri Castl.. The standard molecule for Tilletia indica Mitra contained two DNA fragments, one about 2297 bp DNA sequence from mitochondrial DNA, the other ITS sequence 710 bp. The standard molecule for Tilletia walkeri Castl. contained two DNA fragments, one about 2.3 kb DNA sequence from mitochondrial DNA, the other ITS sequence 710 bp. In this research, the two standard molecules were detected, the results show good specificity, homogeneity and stability which can satisfy the need of molecular detection of Tilletia indica Mitra and Tilletia walkeri Castl..
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LI Xiao-Jiao, WANG Zhong-Kang, ZHANG Gui-Ming, CHEN Zhi-Nan, YIN You-Ping, CHENG Ying-Hui, WANG Ying, XIANG Cai-Yu, MIU Jian-Kun. Construction of Two Standard Molecules for Detection of Tilletia indica Mitra and Tilletia walkeri Castl.[J]. Microbiology China, 2010, 37(10): 1541-1547
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