Comparative Analysis of the Genomes of Foot-and-mouth Disease Virus Isolates from Porcine and Cattle Origin

Abstract:

To investigate the differences between the genomes of foot and mouth viruses isolated from cattle and porcine origin, the complete genomes of two strains of Foot-and-mouth disease virus (FMDV), isolated from clinical tissue samples of pig and cattle, were amplified, sequenced and analyzed. The results showed that the genome sizes of the two viruses were 8236 nt in length, including 38-A poly (A) tail. The complete genomic nucleotide and amino acid identity between the two viruses were above 98.0%-99.4%. The phylogenetic tree based on the amino acid sequences of the entire ORF revealed that the Chinese isolates clustered together with the isolates from GroupⅤ. Our results revealed that the complete genome between the viruses from pig and cattle origin had higher homology and some differences that RGD sequence of the 1D-encoding region of isolates from cattle change into RDD, and the amino acid S change into N or D at +9 site in the isolates from pig. The 3A gene had the genetic characteristics from pig. The results lay a foundation for further study of role of the mutated genes in the molecular basis of fitness variations and changes in host cell tropism.

Key words:FMDV, Complete genome, Integrin, Cell binding site, 3A gene