Diversity of Predominant Bacteria During Ensiling

Abstract:

To analyze the diversity and dynamic changes of predominant bacteria during ensiling, the silage fermented in natural and silage treated with inoculants,which named CK and unit 1 respectively, were sampled in the case of days 0, 1, 3, 5, 15, 30, 60. The pH of each sample was measured and the genomic DNA of microorganism was extracted respectively. After purified by DNA gel Recovery kit, the genomic DNA was subjected to PCR with 16S rDNA (V3 region) primers (341F-GC and 518R). The amplified DNA fragments were separated by denaturing gradient gel electrophoresis (DGGE) and the dominant bands were excised for sequence analysis. The results showed that the pH of unit 1 dropped more rapidly than that of CK, the species of predominant bacteria and numbers of each species also more abundant.