CTAB Method for Genomic DNA Extraction from Nonomuraea

Home > Archive>Volume 37, Issue 8, 2010 >1211-1215

CTAB Method for Genomic DNA Extraction from Nonomuraea
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                        WANG FanWANG Fan
1. Huazhong Agricultural University of Life Science and Technology, Wuhan, Hubei 430070, China
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HONG KuiHONG Kui
1. Huazhong Agricultural University of Life Science and Technology, Wuhan, Hubei 430070, China; 2. Key Laboratory of Tropical Crop Biotechnology, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agri
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Abstract:

In order to obtain genomic DNA which meets the requirements of DNA-DNA hybridization, CTAB method combined with liquid nitrogen grinding was investigated. Cultures from different media added with glycine were harvested in different time. Quality of extracted genomic DNA was assessed by electrophoresis and OD260/OD280 value. Mycelia of shaking culture in yeast extract & malt extract (YE) medium containing 0.3% glycine for 3 days were subjected for DNA extraction. The genomic DNA obtained was about 20 kb, with the OD260/OD280 value around 1.8.

Key words:Nonomuraea, Genomic DNA extraction, CTAB method

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WANG Fan, HONG Kui. CTAB Method for Genomic DNA Extraction from Nonomuraea[J]. Microbiology China, 2010, 37(8): 1211-1215

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