Phylogenetic Diversity of Spore-forming Endophytic Bacteria Isolated from Cinnamomum camphora (Linn.) Presl

Abstract:

Improved NA medium was used to study the diversity of endophytic spore-forming bacteria of Cinnamomum camphora (Linn.) Presl. After spore staining and removing of the redundant isolates, 40 non-redundant spore-forming bacterial isolates were ascertained, which accounted for 29.9% of all the endophytic bacterial isolates, and 25 strains of the 40 were from roots, 5 strains from stems, 10 strains from leaves. Phylogenetic analysis based on 16S rRNA sequences showed that the 40 strains attributed to 12 species of the genera Bacillus, Lysinibacillus, Paenibacillus and Brevibacillus; 7 strains with under 97% sequence similarities respectively to their closely related member were presumed to be potential novel species. Furthermore, microflora of endophytic spore-forming bacteria in roots, stems and leaves of C. camphora showed that some bacteria distributed in different organs, others were organ-specific.

Key words:Cinnamomum camphora, Spore-forming bacteria, Diversity, 16S rRNA