Determination of the Concentration of Biosurfactants Using a Surface Tension Curve Method

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Determination of the Concentration of Biosurfactants Using a Surface Tension Curve Method
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                        LI An-HuaLI An-Hua
1. South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, Guangdong 510650, China; 2. Guangdong Institute of Microbiology, Guangzhou, Guangdong 510070, China; 3. Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangzhou, Guangdong 510070, China; 4. Guangdong Open Laboratory of Applied Microbiology, Guangzhou, Guangdong 510070, China; 5. Graduate School of Chinese Academy of Sciences, Beijing 100049, China
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SUN Guo-PingSUN Guo-Ping
2. Guangdong Institute of Microbiology, Guangzhou, Guangdong 510070, China; 3. Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangzhou, Guangdong 510070, China; 4. Guangdong Open Laboratory of Applied Microbiology, Guangzhou, Guangdong 510070, China
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XU Mei-YingXU Mei-Ying
2. Guangdong Institute of Microbiology, Guangzhou, Guangdong 510070, China; 3. Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangzhou, Guangdong 510070, China; 4. Guangdong Open Laboratory of Applied Microbiology, Guangzhou, Guangdong 510070, China
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Abstract:

In this study, we present the development of a new method for biosurfactants detection. A relation between surface tension and concentration of rhamnolipid was plotted in an x-y scatter by Origin using empirical formula. So the concentration of crude fermentation fluid was determined measuring surface tension of the diluted fermentation fluid with the fitted equation. This method is rapid, simple, accurate and low-cost compared to currently used methods.

Key words:Rhamnolipid, Biosurfactants, Surface tension, Concentration determination

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LI An-Hua, SUN Guo-Ping, XU Mei-Ying. Determination of the Concentration of Biosurfactants Using a Surface Tension Curve Method[J]. Microbiology China, 2010, 37(7): 1083-1087

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