PCR-DGGE Analysis of the Bacterial Community of Chinese Liquor High and Medium Temperature Daqu

Abstract:

The bacterial community structure of 5 high and medium temperature Chinese liquor Daqu were investigated using PCR-DGGE (Polymerase chain reaction-denaturing gradient gel electrophoresis) as a culture-independent method. DNA sequencing was proceeded to obtain the dominant bacterial population information. The result of DGGE profile showed that Weissella cibaria, Lactobacillus helveticus, L. fermentum and L. panis were commonly detected in all the five Daqu. Thermoactinomyces sanguinis was detected only in the Jiangqu sample. Compared with culture-dependent method, DGGE was able to detect Staphylococcus xylosus and Klebsiella oxytoca. The correlation between the craftwork of Daqu and the bacterial community structure was obvious. The Daqu bacterial Shannon-Wiener index decreased as the craftwork temperature of Daqu increasing. PCR-DGGE was proved to be a powerful tool for gaining detailed insight into the bacterial diversity of the Chinese liquor Daqu.