The Selective Enrichment and Diversity Analysis of Halophilic Bacteria in Sedimental Sample from Daban Salt Lake in Xinjiang

Abstract:

The genomic DNA isolated from enriched culture of sedimentary soil samples from Daban Salt Lake in Xinjiang was used as PCR template to amplify 16S rRNA gene and the library carrying 16S rRNA genes of halophilic bacteria was constructed, 100 clones of the library were selected randomly and sequenced for molecular systematic analysis. Phylogenetic analysis indicated that 100 selected clones were clustered into twenty-seven species of nine genera, a lineage of the domain bacteria. Among them, Bacillus was the most dominant genus as represents 48% of the clones, followed by Halobacillus (14%) and Halomonas (13%). Furthermore, ten clones may be potential novel species or genera. The results show that the halophilic bacteria population diversity is abundant in enriched culture of sedimentary soil from Daban Salt Lake in Xinjiang.