Breeding of a High Mizoribine-producing Strain by Composite Mutation with UV and NTG

Abstract:

Eupenicillum sp. E-0509-2 was used as an original strain to carry out mutagenetic breeding adopting UV, NTG and UV + NTG, respectively. The spore suspension of E-0509-2 was treated by UV irradiation for 60 seconds, followed with 3.0 g/L NTG for 20 minuites under magnetic stirring. After further screening experiments on fermentation, a mutant Eupenicillum sp. E-UN41 which was able to produce mizoribine with high yield and could be stably passed on genetics was eventually found. The Mizoribine yield of Eupenicillum sp. E-UN41 was enhanced more than 13 times higher than that of the original strain.

Key words:Mizoribine, Eupenicillum, Composite mutation