Dynamic Changes of Lactic Acid Bacteria During a 15-day Ensilage of Corn

Abstract:

To analyze the dynamic changes of LAB (Lactic acid bacteria) during a 15-day ensilage of corn and the effect that came from inoculants. Plate isolation and 16S rRNA gene homology methods were used to take count of LAB numbers and analyze the population changes during ensilage of corn. One hundred and fifty two LAB were identified as species of Lactobacillus and Pediococcus which were isolated from fermented corn, whereas 86% colonies were identified as Lactobacillus by 16S rRNA gene sequence analysis. The maximum numbers of control group in fermented corn achieved 2.1 × 106 CFU/g and the second experimental group in fermented corn achieved 5.5 × 107 CFU/g after a week. It accelerated growth of Lactobacillus when microbial additive was added. The initial bacteria during ensilage of corn were Pediococcus and Lactobacillus, the dominant bacterium was Lactobacillus in the later phase.

Key words:Ensilage of corn, LAB, Dynamic changes