Characterization of Cold-active Raw Starch-digesting Glucoamylase from Isolated Strain RS01

Abstract:

A strain producing cold-active raw starch-digesting glucoamylase was isolated from Bohai Bay’ samples. The strain RS01 was identified as Aeromonas sp. based on the morphological, physiological characteristics and 16S rRNA sequence. The foundation on the enzymatic properties of partially purified Cold-active RSGA explained that the maximum activity of the enzyme was exhibited at 30°C and pH 5.4. The enzyme thermal stability was poor. The glucose was identified by the TLC, which proved that the strain RS01 has the ability to produce cold-active raw starch-digesting glucoamylase.

Key words:cold-active raw starch-digesting glucoamylase, Aeromonas sp., enzymatic properties