Problems Analysis and Solution Strategies for Fluorescence Observation in VBNC Bacteria

Home > Archive>Volume 37, Issue 5, 2010 >0761-0765

Problems Analysis and Solution Strategies for Fluorescence Observation in VBNC Bacteria
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                        LI YingLI Ying
1. College of Animal Science, Jilin Agriculture University, Changchun, Jilin 130118, China
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DUAN RuiDUAN Rui
2. Liaoyuan Animal Husbandry Stationary, Liaoyuan, Jilin 136200, China
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QIAN Ai-DongQIAN Ai-Dong
1. College of Animal Science, Jilin Agriculture University, Changchun, Jilin 130118, China
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Abstract:

A detection method of viable but non-culturable bacteria by fluorescence microscopy was established with SYTO9 and PI staining cell nucleus, ink instead of Iraq black, specimens fixed by hand. Problems on background fluorescence, cell aggregation, blur fluorescence image, low micropore film absorbability, unclear bacterial form, and fluorescence quenching were analyzed. Some solution strategies were suggested in order to offer help for researchers.

Key words:Viable but non-culturable in bacteria, Fluorescence microscopy, Problems analysis, Solution strategies

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LI Ying, DUAN Rui, QIAN Ai-Dong. Problems Analysis and Solution Strategies for Fluorescence Observation in VBNC Bacteria[J]. Microbiology China, 2010, 37(5): 0761-0765

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