Preliminary Exploration for Inhibitory Mechanism of Bacillus subtilis Strain BS2 to Botrytis Cinerea

Abstract:

The Bacillus subtilis strain 2 (BS2) was measured to antagonistic mechanism by using face to face culturing. The antagonistic effect of BS2 to Botrytis cinerea was obvious. Growth inhibition rate had significant difference between bacteria culture fluid and extracellular proteins at various temperatures (20°C-120°C). But the activity effect of extracellular proteins of BS2 was sensitive to temperature. Bacteria culture fluid and extracellular proteins produced from BS2 had a good antibacterial effect on B. cinerea mycelial growth, spores bearing and germination. And Treated with bacteria culture fluid and extracellular proteins produced from BS2, protoplast from the hyphae became abnormal. Overall, this study indicates that the BS2 active substance is composed of various components and has multiple antagonistic mechanism.